Welp…
Fauci and friends now working on Spanish Flu gain of function research…
tomrenz.substack.com/p/fauci-is-now-performing-gain-of-function
Fauci is Now Performing Gain-of-Function on the Spanish Flu
Tom Renz
17 hr ago
This will be short because it really does not need much comment. In fact, this is so absurd that I am just starting with the reference document because I am concerned no one will believe it. Here it is:
Spanish Flu Gof
tomrenz.substack.com/api/v1/file/349d79f6-d3bc-4f89-b0bb-f2b8151b74c5.pdf
Yes, that is right, Fauci and crew are now actively performing gain-of-function (GoF) work and infecting primates with the Spanish Flu. For those of you that are unaware, GoF does not have a single agreed upon definition but, as it relates here, is essentially the modification of the Spanish Flu virus to make “more functional.” In this case, as with COVID, I have little doubt the GoF supporters will argue that this is not GoF but the article actually notes that this disease was created in canine kidneys with supplemental bovine serum. Here is a quote from the document:
Virus and cells. Influenza virus A/South Carolina/1918 (H1N1) was generated by reverse genetics (9) and handled in biosafety level 4 (BSL-4) containment at the National Microbiology Laboratory (NML). Sequences of the 1918 influenza viral segments were based on data reported under GenBank accession numbers DQ208309, DQ208310, DQ208311, AF117241, AY744935, AF250356, AY130766, and AF333238. 1918 influenza virus was cultured usingMadin-Darby canine kidney (MDCK; ATCC, Manassas, VA, USA) cells. MDCK cells were grown in minimum essential medium (MEM; HyClone) supplemented with 5% fetal bovine serum (FBS; HyClone) and 1 L-glutamine (L-Glu; Gibco, Life Technologies, Grand Island, NY, USA).
A passage 2 (P2) virus stock was prepared using MEM supplemented with 0.1% bovine serum albumin (BSA) (fraction V; HyClone), 1 L-glutamine, and 1 mg/mL N-tosyl-L-phenylalanine chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich). This stock was used for animal inoculation. The mouse 50% lethal dose (MLD50) for this stock was determined previously to be 103.2 PFU (9); this value was confirmed prior to the use of the stock for macaque infection.
LOTS MORE AT LINK
h/t Alchemike